Atomic Force Microscopy Facility

Equipment

JPK NanoWizard 4XP

Our system consists of a JPK NanoWizard 4XP mounted on an inverted Zeiss Axio Observer optical microscope with fluorescence. The use of a motorized precision stage allows for easy sample navigation and precise positioning. When used in conjunction with DirectTiling, it can image large areas for feature identification and overlay.

The NanoWizard 4 XP is a flexible system that combines atomic resolution and fast scanning with rates of up to 150 lines/sec and a large scan range of 100µm.

Available modes:

• Contact Mode Imaging
• Tapping Mode Imaging
• PeakForce Tapping Imaging
• Quantitative Imaging
• Frequency Modulated Imaging
• Fast Scan Imaging
• Force Spectroscopy
• Force Mapping
• Single-Cell Force Spectroscopy
• MicroRheology

CellHesion 200 AFM

A separate JPK CellHesion 200 AFM head is available for measuring cell-cell, cell-substrate and colloid-surface interactions. This system gives an extended z-length of 100µm, particularly suited to investigate cellular adhesion dynamics including maximum cell adhesion force, unbinding events and tether characteristics.

Accessory Modules

• PetriDish Heater
  • Allows temperature range between RT and 60oC for liquid experiments.
  • Ports for perfusion tubes.
• Heating Cooling Module
  • Temperature range between -30 to 120oC in air.
  • Temperature range of 0-80oC in liquid.
• Coverslip holder
  • Closed liquid cell with coverslip bottom.
  • Allows for small volume experiments and optical clarity for fluorescent imaging.
• Cytosurge FluidFM
  • Hollow AFM probes with the ability to handle femtoliter liquid volumes.
  • Micropipette – single cell and colloidal spectroscopy.
  • Nanosyringe – single-cell injection/extraction.
  • Nanopipette – Bacteria adhesion/nanoprinting.