Synthetic genetic circuits are the foundational technology that permit mammalian cells to be transformed into agents, be that for bioproduction, tissue engineering or cell therapies. Given their importance, it is our inconvenient truth that we are yet to achieve sufficient control of their performance1, nor the ability to scale their design beyond a few devices2 or ensure their long-term operation3.This PhD will dive deep into the parameters that influence synthetic genetic circuits and work to redefine the boundaries of the possible. You’ll become expert in the genetic modification of mammalian cells (from lines to primary and stem cells) using a huge range of different transfection approaches and a host of analytical approaches from advanced imaging and molecular biology. The work benefits from the rich environment of the University of Edinburgh and core facilities such as the Edinburgh Genome Foundry and Edinburgh Genomics.Engineering Biology is developing rapidly as a preeminent technology for the delivery of much of humankind’s needs and this PhD will equip you to contribute to diverse fields from cultured meat to therapeutic cells. More background is available by contacting Prof. Alistair Elfick.Yeoh et al. (2022) Genetic Circuit Design Principles. In: Thouand, G. (eds) Handbook of Cell Biosensors. Springer, Cham.Shakiba et al. (2021) Context-aware Synthetic Biology by Controller Design: Engineering the Mammalian Cell, Cell Systems, 12(6), 561-592.Cabrera et al. (2022) The sound of silence: Transgene silencing in mammalian cell engineering, Cell Systems, 13(12), 950-973. Further information The University of Edinburgh is committed to equality of opportunity for all its staff and students, and promotes a culture of inclusivity: https://www.ed.ac.uk/equality-diversity Closing date:  Fri, 28/02/2025 - 12:00 Apply now Principal Supervisor Professor Alistair Elfick Assistant Supervisor Dr Lucia Bandiera Eligibility Minimum entry qualification - an Honours degree at 2:1 or above (or International equivalent) in a relevant science or engineering discipline, possibly supported by an MSc Degree. Further information on English language requirements for EU/Overseas applicants. Funding Applications are welcomed from self-funded students, or students who are applying for scholarships from the University of Edinburgh* or elsewhere.Further information and other funding options.*A number of scholarships are available through a competitive process. Applicants are encouraged to contact the project’s supervisor, Professor Elfick, to discuss their interest in applying for the project, and scholarships. Informal Enquiries Alistair.Elfick@ed.ac.uk